Pepinh-TRIF

TRIF inhibitory peptide

Pepinh-TRIF is a 30 aa peptide that blocks TRIF signaling by interfering with TLR-TRIF interaction.

PepinhTRIF contains the 14 aa that correspond to the sequence of the BB loop of TRIF (FCEEFQVPGRGELH) [1] linked to the cell-penetrating segment of the antennapedia homoedomain (RQIKIWFQNRRMKWKK) [2].

Pepinh TRIF is provided with Pepinh-Control, a control peptide [1].

References:

1. Toshchakov V. et al., 2005. Differential Involvement of BB Loops of Toll-IL-1 Resistance (TIR) Domain-Containing Adapter Proteins in TLR4- versus TLR2-Mediated Signal Transduction. J. Immunol., 175: 494 - 500.2. Derossi D. et al., 1994. The third helix of the Antennapedia homeodomain translocates through biological membranes. J. Biol. Chem., 269: 10444-50.

Specifications

Working concentration: 5-50 μM

Purity:  ≥95% (UHPLC)

Species reactivity: Human, mouse

Sequences:

• Pepinh-TRIF: RQIKIWFQNRRMKWKK-FCEEFQVPGRGELH-NH2

(TRIF homodimerization sequence is underlined)

• Pepinh-Control: RQIKIWFQNRRMKWKK-SLHGRGDPMEAFII-NH2

Molecular weights:

• Pepinh-TRIF: 3918
• Pepinh-Control: 3812

Contents

Pepinh-TRIF is provided as a white solid .

• 2 mg of lyophilized Pepinh-TRIF
• 2 mg of lyophilized Pepinh-Control
• 2x1.5 ml endotoxin-free water

Pepinh-TRIF is shipped at room temperature.

Store at -20°C.

Solid product is stable 1 year at -20°C.

Upon resuspension, Pepinh-TRIF should be aliquoted and stored at -20°C.

Avoid repeated freeze-thaw cycles.

Resuspended product is stable for 6-8 months at -20°C when properly stored.