请使用支持JavaScript的浏览器!
主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
InvivoGen/LPS-RS/tlrl-rslps
产品编号:tlrl-rslps
市  场 价:¥53480.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2674.00
品      牌: InvivoGen
公      司:InvivoGen
公司分类:
InvivoGen/LPS-RS/tlrl-rslps
商品介绍

LPS-RS

LPS-RSUnit sizeCat. codeDocsPrice
LPS from R. sphaeroides
5 mg
tlrl-rslps
TDSMSDS
¥2,674.00
Please contact our distributor
Add to favorite
LPS-RS UltrapureUnit sizeCat. codeDocsPrice
Ultrapure lipopolysaccharide from R. sphaeroides
1 mg
tlrl-prslps
TDSMSDS
¥2,501.00
Please contact our distributor
Add to favorite
  • About
  • Specifications
  • Contents
  • Description
  • Citations

LPS from R. sphaeroides

Lipopolysaccharide from the photosynthetic bacterium Rhodobacter sphaeroides (LPS-RS) is a potent antagonist of lipopolysaccharide (LPS) from pathogenic bacteria [1]. Complete competitive inhibition of LPS activity is possible at a 100-fold excess of the antagonist. LPS-RS does not induce TLR4 signaling but is detected by the LAL assay, the standard endotoxin detection assay.

InvivoGen provides LPS-RS with two grades of purity:

  • LPS-RS is a standard lipopolysaccharide (LPS) preparation extracted by a phenol-water mixture. It inhibits TLR4 activity, however, as LPS-RS contains other bacterial components, such as lipoproteins, it activates TLR2.
  • LPS-RS Ultrapure is extracted by successive enzymatic hydrolysis steps and purified by the previously described phenol-TEA-DOC extraction protocol [2]. This process removes contaminating lipoproteins, and therefore LPS-RS Ultrapure does not activate TLR2 while retaining the ability to inhibit TLR4 activity. 

 

Reference:

1. Coats SR. et al., 2005. MD-2 mediates the ability of tetra-acylated and penta-acylated lipopolysaccharides to antagonize Escherichia coli lipopolysaccharide at the TLR4 signaling complex. J Immunol.;175(7):4490-8.2. Hirschfeld M. et al., 2000. Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol. 165(2):618-22.

Back to the top

Specifications

Specificity: TLR4 antagonistLPS-RS standard is also a TLR2 agonist

Working concentration: 10 ng - 10 μg/ml

Endotoxin level: 1  x 106 EU/mg

Solubility: 5 mg/ml in water

InvivoGen provides LPS-RS with two grades of purity: Standard and Ultrapure

Back to the top

Contents

LPS-RS:

  • 5 mg lipopolysaccharide from Rhodobacter sphaeroides (LPS-RS)
  • 1.5 ml endotoxin-free water

LPS-RS Ultrapure:

  • 1 mg lipopolysaccharide from Rhodobacter sphaeroides  Ultrapure (LPS-RS Ultrapure)
  • 1.5 ml endotoxin-free water

LPS-RS is shipped at room temperature

Upon receipt it should be stored at -20 ̊C.Upon resuspension, prepare aliquots of LPS-RS and store at 4°C for short term storage or -20 ̊C for long term storage.

Lyophilized product is stable 1 year at -20°C when properly stored.Resuspended product is stable 1 month at 4°C and 6 months at -20°C.

Avoid repeated freeze-thaw cycles.

Back to the top

Description

LPS from the photosynthetic bacterium Rhodobacter sphaeroides (LPS-RS) is the first potent antagonist of toxic LPS in both human and murine cells and also prevents LPS-induced shock in mice [1].

LPS-RS is penta-acylated and as other under-acylated LPS seems to utilize at least two distinct mechanisms to block LPS-dependent activation of TLR4.

The main mechanism consists of direct competition between under-acylated LPS and hexa-acylated LPS for the same binding site on MD-2, while the secondary mechanism involves the ability of under-acylated LPS:MD-2 complexes to inhibit hexa-acylated endotoxin: MD-2 complexes function at TLR4 [2-5].

Complete competitive inhibition of LPS activity is possible at a 100 fold excess of the antagonist. LPS-RS does not induce TLR4 signaling but is detected by the LAL assay, the standard endotoxin detection assay. 

LPS-RS preparations (cat. code tlrl-rslps) contain lipopeptide contaminants, and therefore stimulate Toll-like receptor 2 (TLR2).

 

1. Qureshi, N. et al., 1999. Nontoxic RsDPLA as a potent antagonist of toxic lipopolysaccharide. p. 687-698. In: H. Brade, and S. M. Opal, and S. N. Vogel, and D. C. Morrison, eds. Endotoxin in Health and Disease 687. Marcel Dekker, New York.2. Coats SR. et al., 2005. MD-2 mediates the ability of tetra-acylated and penta-acylated lipopolysaccharides to antagonize Escherichia coli lipopolysaccharide at the TLR4 signaling complex.J Immunol.;175(7):4490-8.3. Teghanemt A. et al., 2005. Molecular basis of reduced potency of underacylated endotoxins. J Immunol. 175(7):4669-76.4. Visintin A. et al., 2005. Pharmacological inhibition of endotoxin responses is achieved by targeting the TLR4 coreceptor, MD-2. J Immunol. 175(10):6465-72.5. Saitoh S. et al., 2004. Lipid A antagonist, lipid IVa, is distinct from lipid A in interaction with Toll-like receptor 4 (TLR4)-MD-2 and ligand-induced TLR4 oligomerization. Int Immunol. 16(7):961-9.

Back to the top

Citations

Role of toll-like receptor 4 antagonist Lipopolysaccharide-Rhodobacter sphaeroides on acute stress-induced voluntary ethanol preference and drinking behaviour: In vivo Swiss Albino mouse model.

2020 Eur Neuropsychopharmacol. DOI: 10.1016/j.euroneuro.2019.12.121

Role of toll-like receptor 4 antagonist Lipopolysaccharide-Rhodobacter sphaeroides on acute stress-induced voluntary ethanol preference and drinking behaviour: In vivo Swiss Albino mouse model.

Chuang H.G. et al.

Toll-like receptor 4 agonist and antagonist lipopolysaccharides modify innate immune response in rat brain circumventricular organs.

2020 J Neuroinflammation. DOI: 10.1186/s12974-019-1690-2

Toll-like receptor 4 agonist and antagonist lipopolysaccharides modify innate immune response in rat brain circumventricular organs.

Vargas-Caraveo A. et al.

Effect of ultrapure lipopolysaccharides derived from diverse bacterial species on the modulation of platelet activation.

2019 Sci Rep. DOI: 10.1038/s41598-019-54617-w

Effect of ultrapure lipopolysaccharides derived from diverse bacterial species on the modulation of platelet activation.

Vallance T.M. et al.

Serum FHR1 binding to necrotic-type cells activates monocytic inflammasome and marks necrotic sites in vasculopathies.

2019 Nat Commun. DOI: 10.1038/s41467-019-10766-0

Serum FHR1 binding to necrotic-type cells activates monocytic inflammasome and marks necrotic sites in vasculopathies.

Irmscher S. et al.

Specific features of human monocytes activation by monophosphoryl lipid A.

2018 Sci Rep. 8(1):7096.

Specific features of human monocytes activation by monophosphoryl lipid A.

Chentouh R. et al.

Connections of annexin A1 and translocator protein-18 kDa on toll like receptor stimulated BV-2 cells.

2018 Exp Cell Res. 367(2):282-290.

Connections of annexin A1 and translocator protein-18 kDa on toll like receptor stimulated BV-2 cells.

Pantaleão L. et al.

Bovine lactoferrin reduces extra-territorial facial allodynia/hyperalgesia following a trigeminal nerve injury in the rat.

2017 Brain Res. S0006-8993(17)30176-2.

Bovine lactoferrin reduces extra-territorial facial allodynia/hyperalgesia following a trigeminal nerve injury in the rat.

Horie K. et al.

PKC-δ isoform plays a crucial role in Tat-TLR4 signalling pathway to activate NF-κB and CXCL8 production.

2017 Sci Rep. 7(1):2384.

PKC-δ isoform plays a crucial role in Tat-TLR4 signalling pathway to activate NF-κB and CXCL8 production.

Serrero M. et al.

Site-specific regulation of P2X7 receptor function in microglia gates morphine analgesic tolerance.

2017 J Neurosci. 37(42):10154-10172.

Site-specific regulation of P2X7 receptor function in microglia gates morphine analgesic tolerance.

Leduc-Pessah H. et al.

Dengue virus NS1 protein activates immune cells via TLR4 but not TLR2 or TLR6.

2017 Immunol Cell Biol. 95(5):491-495.

Dengue virus NS1 protein activates immune cells via TLR4 but not TLR2 or TLR6.

Modhiran N. et al.

Soluble CD14 acts as a DAMP in human macrophages: origin and involvement in inflammatory cytokine/chemokine production.

2017 FASEB J. 31(5):1891-1902.

Soluble CD14 acts as a DAMP in human macrophages: origin and involvement in inflammatory cytokine/chemokine production.

Lévêque M. et al.

Pathogen-mimicking vaccine delivery system designed with a bioactive polymer (inulin acetate) for robust humoral and cellular immune responses.

2017 J Control Release. S0168-3659(17)30690-9.

Pathogen-mimicking vaccine delivery system designed with a bioactive polymer (inulin acetate) for robust humoral and cellular immune responses.

Tummala H. et al.

Ebolaviruses associated with differential pathogenicity induce distinct host responses in human macrophages.

2017 J Virol. JVI.00179-17.

Ebolaviruses associated with differential pathogenicity induce distinct host responses in human macrophages.

Olejnik J. et al.

Lipopolysaccharide induces proliferation and osteogenic differentiation of adipose-derived mesenchymal stromal cells in vitro via TLR4 activation.

2016 Exp Cell Res. S0014-4827(16)30385-8.

Lipopolysaccharide induces proliferation and osteogenic differentiation of adipose-derived mesenchymal stromal cells in vitro via TLR4 activation.

Herzmann N. et al.

MSRV envelope protein is a potent, endogenous and pathogenic agonist of human toll-like receptor 4: Relevance of GNbAC1 in multiple sclerosis treatment.

2016 J Neuroimmunol. 291:29-38.

MSRV envelope protein is a potent, endogenous and pathogenic agonist of human toll-like receptor 4: Relevance of GNbAC1 in multiple sclerosis treatment.

Madeira A, Burgelin I, Perron H, Curtin 2, Lang AB, Faucard R.

Antiphospholipid antibody-induced miR-146a-3p drives trophoblast interleukin-8 secretion through activation of Toll-like receptor 8.

2016 Mol Hum Reprod. 22(7):465-74.

Antiphospholipid antibody-induced miR-146a-3p drives trophoblast interleukin-8 secretion through activation of Toll-like receptor 8.

Gysler SM. et al.

Circulating exosomes from patients with systemic lupus erythematosus induce an proinflammatory immune response.

2016 Arthritis Res Ther. 18(1):264.

Circulating exosomes from patients with systemic lupus erythematosus induce an proinflammatory immune response.

Lee JY. et al.

Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.

2015 Infect Immun. 83(1):227-38.

Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.

Brummelman J, Veerman RE, Hamstra HJ, Deuss AJ, Schuijt TJ, Sloots A, Kuipers B, van Els CA, van der Ley P, Mooi FR, Han WG, Pinelli E.

Dengue virus NS1 protein activates cells via Toll-like receptor 4 and disrupts endothelial cell monolayer integrity.

2015 Sci Transl Med. 7(304):304ra142.

Dengue virus NS1 protein activates cells via Toll-like receptor 4 and disrupts endothelial cell monolayer integrity.

Modhiran N, Watterson D, Muller DA, Panetta AK, Sester DP, Liu L, Hume DA, Stacey KJ, Young PR.

Toll-like receptor 4 signaling contributes to paclitaxel-induced peripheral neuropathy.

2014 J Pain. pii: S1526-5900(14)00678-6

Toll-like receptor 4 signaling contributes to paclitaxel-induced peripheral neuropathy.

Li Y, Zhang H, Zhang H, Kosturakis AK, Jawad AB, Dougherty PM

TLR4 antagonist reduces early-stage atherosclerosis in diabetic apolipoprotein E-deficient mice.

2013 J Endocrinol.216(1):61-71.

TLR4 antagonist reduces early-stage atherosclerosis in diabetic apolipoprotein E-deficient mice.

Lu Z, Zhang X, Li Y, Jin J, Huang Y.

Inhaled birch pollen extract induces airway hyperresponsiveness via oxidative stress but independently of pollen-intrinsic NADPH oxidase activity, or the TLR4-TRIF pathway.

2013 J Immunol. 191(2):922-33

Inhaled birch pollen extract induces airway hyperresponsiveness via oxidative stress but independently of pollen-intrinsic NADPH oxidase activity, or the TLR4-TRIF pathway.

Shalaby KH, Allard-Coutu A, O'Sullivan MJ, Nakada E, Qureshi ST, Day BJ, Martin JG.

Follistatin-related protein/follistatin-like 1 evokes an innate immune response via CD14 and toll-like-receptor 4.

2012 FEBS Lett. 586(4):319-24

Follistatin-related protein/follistatin-like 1 evokes an innate immune response via CD14 and toll-like-receptor 4.

Murakami K, Tanaka M, Usui T, Kawabata D, Shiomi A, Iguchi-Hashimoto M, Shimizu M, Yukawa N, Yoshifuji H, Nojima T, Ohmura K, Fujii T, Umehara H, Mimori T

Load more
品牌介绍

Invivogen


InvivoGen的主要重点是通过提供创新的研究工具,促进生命科学的进步。除了我们的先天免疫研究产品,我们利用在发酵和克隆方面的技术,提供高质量的抗生素和新型质粒。我们提供所有常见的抗生素和哺乳动物选择抗生素,以预防和消除所有类型的微生物污染物,包括支原体等。我们也是开发和销售无CpG质粒的唯一公司。我们为研究团体提供多种产品系列,涉及许多学科,包括RNA干扰,免疫球蛋白A,基因治疗和癌症研究。


InvivoGen was founded in 1997 by scientists with technical expertise and creative excellence stemming from a longstanding history in microbiology. InvivoGen's unparalleled skill in microbial fermentation enables us to provide a wide range of biological molecules, including ultra-pure antibiotics, novel mycoplasma treatments and the largest collection of pattern recognition receptor agonists derived from a wide range of micro-organisms.


In addition we boast strong chemistry and molecular biology teams for the synthesis of molecules and plasmids for research use and studies focused on gene therapy and vaccination. Moreover, our specialized study of the innate immune receptor signaling pathways has resulted in the collection of reporter cell lines, which are routinely used for in-house quality control and research and development.


产品列表:


Invivogen

ant-agl-25

17-AAG

25 mg

Invivogen

ant-agl-5

17-AAG

5 mg

Invivogen

ant-egl-1

17-AEP-GA

1 mg

Invivogen

ant-egl-5

17-AEP-GA

5 mg

Invivogen

ant-dgl-25

17-DMAG

25 mg

Invivogen

ant-dgl-5

17-DMAG

5 mg

Invivogen

ant-mgl-1

17-DMAP-GA

1 mg

Invivogen

ant-mgl-5

17-DMAP-GA

5 mg

Invivogen

gmbapa-ga

17-GMB-APA-GA

1 mg

Invivogen

ant-nhgl-1

17-NHS-ALA-GA

1 mg

Invivogen

tlrl-nacda23

2'3'-c-di-AMP

500 µg

Invivogen

tlrl-25gpap

2'3'-cGAMP Control

1 mg

Invivogen

tlrl-apr

2-Aminopurine

250 mg

Invivogen

293-hmd2cd14

293/hMD2-CD14

3-7 x 10e6 cells

Invivogen

293-hnod1

293/hNOD1

3-7 x 10e6 cells

Invivogen

293-hnod2

293/hNOD2

3-7 x 10e6 cells

Invivogen

293-htlr1ha

293/hTLR1-HA

3-7 x 10e6 cells

Invivogen

293-htlr10ha

293/hTLR10-HA

3-7 x 10e6 cells

Invivogen

293-htlr2

293/hTLR2

3-7 x 10e6 cells

Invivogen

293-htlr2cd14

293/hTLR2-CD14

3-7 x 10e6 cells

Invivogen

293-htlr2ha

293/hTLR2-HA

3-7 x 10e6 cells

Invivogen

293-htlr2/6

293/hTLR2-TLR6

3-7 x 10e6 cells

Invivogen

293-htlr3

293/hTLR3

3-7 x 10e6 cells

Invivogen

293-htlr3ha

293/hTLR3-HA

3-7 x 10e6 cells

Invivogen

293-htlr4ha

293/hTLR4-HA

3-7 x 10e6 cells

Invivogen

293-htlr4a

293/hTLR4A

3-7 x 10e6 cells

Invivogen

293-htlr4md2cd14

293/hTLR4A-MD2-CD14

3-7 x 10e6 cells

Invivogen

293-htlr5

293/hTLR5

3-7 x 10e6 cells

Invivogen

293-htlr5cd14

293/hTLR5-CD14

3-7 x 10e6 cells

Invivogen

293-htlr5ha

293/hTLR5-HA

3-7 x 10e6 cells

Invivogen

293-htlr6ha

293/hTLR6-HA

3-7 x 10e6 cells

Invivogen

293-lacz

293/LacZ

3-7 x 10e6 cells

Invivogen

293-mnod1

293/mNOD1

3-7 x 10e6 cells

Invivogen

293-mnod2

293/mNOD2

3-7 x 10e6 cells


自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔