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℡ 4000-520-616
℡ 4000-520-616
InvivoGen/Murine Anti-mCTLA4 mAb/mctla4-mab10-1
产品编号:mctla4-mab10-1
市  场 价:¥91720.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
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美  元  价:$4586.00
品      牌: InvivoGen
公      司:InvivoGen
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InvivoGen/Murine Anti-mCTLA4 mAb/mctla4-mab10-1
商品介绍

Murine Anti-mCTLA4 mAb

Anti-mCTLA4-mIgG2a InvivoFit™Unit sizeCat. codeDocsPrice
9D9-derived mouse monoclonal antibody against murine CTLA-4
1 mg
10 mg
mctla4-mab10-1
TDSMSDSDATA
¥4,586.00
Please contact our distributor
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  • About
  • Specifications
  • Contents
  • Details
  • Related products

Recombinant mouse mAb against murine CTLA-4 for in vivo use

Effect of Anti-CTLA4 on T cell functionEffect of Anti-CTLA4 on T cell activation

Anti-mCTLA4-mIgG2a InvivoFit™ is a mouse anti-mouse monoclonal antibody (mAb) featuring the variable region of the previously described anti-mCTLA-4 9D9 mAb [1] and a murine IgG2a constant region. The 9D9 mAb has a sequence that is 100% murine (constant and variable regions), ensuring minimal immunogenicity upon repeated injections into mice. This mAb targets murine cytotoxic T-lymphocyte-associated protein 4 (mCTLA-4) and blocks CTLA4-mediated negative signals downregulating T cell activation.  Thus, anti-mCTLA-4 mAb unleashes T cell activity [1, 2].Notably, the 9D9 mAb, along with most commercially available anti-mCTLA4 mAbs feature the IgG2b isotype which displays low antitumor activity in vivo [2]. To overcome this issue, Anti-mCTLA4-mIgG2a InvivoFit™ was generated by recombinant technology and features the murine IgG2a isotype, which mediates potent cytotoxic functions. Anti-mCTLA4-mIgG2a is provided in an InvivoFit grade, a high-quality standard specifically adapted to in vivo studies.

 

Key features of Anti-mCTLA4-mIgG2a InvivoFit™:

  • Sequence is 100% murine
  • Features the IgG2a isotype (associated with high antitumor activity)
  • Guaranteed sterile, endotoxin level < 1 EU/mg
  • Specifically designed for in vivo studies in mice
  • Low aggregation < 5%
  • Produced in both animal-free facilities and defined media

Anti-mCTLA4-mIgG2a InvivoFit™ is produced in Chinese hamster ovary (CHO) cells, purified by affinity chromatography with protein A, and it"s binding to mCTLA-4 is validated using ELISA.

 

References:

1. Selby MJ. et al., 2013. Anti-CTLA-4 antibodies of IgG2a isotype enhance antitumor activity through reduction of intratumoral regulatory T cells. Cancer Immunol Res. 1(1):32-42.2. Ribas A. & Wolchock J.D., 2018. Cancer immunotherapy using checkpoint blockade. Science. 359:1350-55.

Figures

Evaluation of Anti-mCTLA4-mIgG2a binding to mCTLA-4
Evaluation of Anti-mCTLA4-mIgG2a binding to mCTLA-4

ELISA binding of Anti-mCTLA4-mIgG2a InvivoFit™ to recombinant murine CTLA-4.

A dilution series of Anti-mCTLA4-mIgG2a InvivoFit™ and anti-mCTLA4 (9D9 clone) was used for the detection of coated mCTLA-4 antigen at 5  µg/ml. Detection of bound antibody was performed using an HRP‑conjugated anti‑murine κ-light chain secondary antibody.  HRP activity was assessed by a colorimetric assay using Tetramethylbenzidine (TMB) substrate. Optical density was measured at 490 nm. Data are shown as percentage absorbance.

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Specifications

Specificity: Targets cells expressing murine CTLA-4

Formulation: Lyophilized from 0.2 μm filtered solution in 150 mM sodium chloride, 20 mM sodium phosphate buffer with 5% saccharose

Clonality: Monoclonal antibody

Isotype: Murine IgG2a, kappa

Source: CHO cells

Purity: Purified by affinity chromatography with protein A

Tested application: ELISA

Quality control:

  • Binding confirmed using ELISA
  • The complete sequence of this antibody has been verified
  • < 5% aggregates (confirmed by size exclusion chromatography)
  • Guaranteed sterile and <1 EU/mg (determined by the LAL assay)
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Contents

Anti-mCTLA4-mIgG2a InvivoFit™ is provided sterile, endotoxin-free, azide-free and lyophilized.  

This product is available in two pack sizes:

  • mctla4-mab10-1​: 1 mg
  • mctla4-mab10-10​: 10 mg

 

room temperature Product is shipped at room temperature.

store Store lyophilized antibody at -20 °C.

stability Lyophilized product is stable for at least 1 year

Alert Avoid repeated freeze-thaw cycles.

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Details

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4, also known as CD152) is an inhibitory receptor expressed on activated T lymphocytes. T-cell activation requires two signals. Signal 1 is delivered upon interaction of the T cell receptor (TCR) with antigenic peptides bound to major histocompatibility complex (MHC) molecules on antigen-presenting cells (APCs). Signal 2 is delivered upon interaction of the co-stimulatory receptor CD28 with the B7 family ligands, B7-1 (CD80) and B7-2 (CD86), on APCs.CTLA-4 is an immune checkpoint molecule upregulated upon T cell activation. It exerts competitive binding to CD28 ligands, thereby preventing T-cell overstimulation. CTLA-4 blockade with monoclonal antibodies (mAbs) has been used to treat patients with metastatic melanoma.

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You may also need

Raji-hCTLA4 Cells

Human lymphoblast cells - ADCC CTLA-4 Target Cells

Anti-hCTLA4 isotype family

Family of antibodies against human CTLA-4

Mouse IgG2a Control

β-galactosidase antibody (isotype control) - Mouse IgG2a

品牌介绍

Invivogen


InvivoGen的主要重点是通过提供创新的研究工具,促进生命科学的进步。除了我们的先天免疫研究产品,我们利用在发酵和克隆方面的技术,提供高质量的抗生素和新型质粒。我们提供所有常见的抗生素和哺乳动物选择抗生素,以预防和消除所有类型的微生物污染物,包括支原体等。我们也是开发和销售无CpG质粒的唯一公司。我们为研究团体提供多种产品系列,涉及许多学科,包括RNA干扰,免疫球蛋白A,基因治疗和癌症研究。


InvivoGen was founded in 1997 by scientists with technical expertise and creative excellence stemming from a longstanding history in microbiology. InvivoGen's unparalleled skill in microbial fermentation enables us to provide a wide range of biological molecules, including ultra-pure antibiotics, novel mycoplasma treatments and the largest collection of pattern recognition receptor agonists derived from a wide range of micro-organisms.


In addition we boast strong chemistry and molecular biology teams for the synthesis of molecules and plasmids for research use and studies focused on gene therapy and vaccination. Moreover, our specialized study of the innate immune receptor signaling pathways has resulted in the collection of reporter cell lines, which are routinely used for in-house quality control and research and development.


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